Bcells Clusters

Gene Expression

Kappa vs Lambda Expression

Igs Expression

Other Genes’ Expression

Clusters’ Gene Markers

Cluster 0

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
GPR183 1.3872860905509 0.643 0.321 1.50093013586677e-293
CXCR4 0.897002084782664 0.946 0.646 2.79465114456748e-269
NR4A2 1.01120472991501 0.58 0.339 1.57297953329415e-168
ARHGAP24 0.951281223665877 0.454 0.209 9.03405876935521e-167
CD83 0.80962903633932 0.596 0.359 2.31508248081161e-135
REL 0.843537344418049 0.52 0.301 8.6653603382112e-117
LY9 0.82689440420669 0.401 0.241 7.02464835412563e-77
CREM 0.943289403623355 0.372 0.295 6.09109551075867e-27

Cluster 1

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHD 2.26937422631391 0.636 0.072 0
TCL1A 1.7630542485369 0.58 0.1 0
FCER2 1.64147629158705 0.492 0.088 0
BTG1 1.22546167629801 0.986 0.881 0
CD72 1.17805125124466 0.372 0.091 0
CXCR4 1.16152267294715 0.964 0.645 0
HLA-DRB1 1.12500543899733 0.924 0.682 0
CD52 1.11943294122547 0.957 0.699 0
LAPTM5 1.11238598612084 0.892 0.596 0
HLA-DPA1 0.982993408017732 0.951 0.714 0
HLA-DPB1 0.949419931075115 0.957 0.698 0
HLA-DRA 0.940153958621598 0.998 0.795 0
YBX3 1.2467485659192 0.31 0.08 4.48455805454258e-253
HLA-DQA1 0.977022935929604 0.841 0.584 9.28421960879641e-248
IL4R 1.06495586857944 0.261 0.065 4.80146387161673e-208
SELL 0.991289417372965 0.416 0.18 2.73692488119944e-160
RHOH 0.970306103581982 0.613 0.427 3.30046781677669e-144
STAG3 1.01814473289099 0.291 0.11 2.55554256341291e-130
SARAF 1.05688550927337 0.527 0.313 5.60938809293103e-128
CHMP1B 1.13859186116654 0.388 0.231 2.96314808802244e-83

Cluster 2

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
RP5-887A10.1 1.49968785886481 0.624 0.203 0
RPS29 0.939276659481525 0.998 0.951 0
RPS12 0.928704871719422 1 0.985 0
RPL38 0.897563425097028 0.995 0.927 0
RPL39 0.869893889866293 0.996 0.901 0
RPS21 0.859690733291504 0.994 0.924 0
RPL31 0.824896983932141 1 0.974 0
RPL30 0.82108216047948 1 0.969 0
RPLP2 0.819383349060717 1 0.992 0
RPS27 0.812621288758966 1 0.991 0
TOMM7 0.817238749661796 0.959 0.813 3.70253350907267e-236

Cluster 3

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
AC096579.7 3.40185614921032 0.572 0.128 0
IGKC 3.3591239326899 0.996 0.916 0
IGHA2 3.16260146988569 0.97 0.502 0
IGHA1 2.30823974363442 0.998 0.844 0
HSPA1B 2.02938341373645 0.703 0.294 0
HERPUD1 1.45159797626684 0.941 0.735 0
DERL3 1.4436639512233 0.767 0.326 0
SSR4 1.37709447993418 0.969 0.671 0
MZB1 1.35426809163824 0.946 0.412 0
FKBP11 1.33040682452385 0.761 0.307 0
JUN 1.6746957451251 0.905 0.695 3.55632562274781e-291
TIMP1 1.31134621758947 0.587 0.239 5.13874886366238e-258
AL928768.3 1.44027172669107 0.774 0.401 5.08508130151852e-246
DNAJB9 1.34276272655493 0.69 0.342 7.97051016225126e-244
HSPA1A 1.72537412715778 0.61 0.305 3.91131484789896e-209
CITED2 1.3161938486866 0.532 0.249 5.69973293932676e-171
HSPA5 1.32116832808557 0.636 0.377 2.4446798274215e-158
IER2 1.50598642084791 0.743 0.542 1.10956700929047e-141
RGCC 1.38600252789561 0.488 0.281 1.85332678531166e-87
JCHAIN 2.44362074120798 0.301 0.315 0.0024070944722449

Cluster 4

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HLA-DPB1 1.2950921450342 0.983 0.709 0
HLA-DPA1 1.25308704746769 0.976 0.723 0
HLA-DRA 1.14090035178467 0.999 0.805 0
CD74 1.09885364846992 1 0.957 0
TMSB4X 0.911356013287482 1 0.992 9.33778827268325e-277
HLA-A 1.08157550050729 0.981 0.886 1.1981664192463e-263
ACTB 0.925010927903783 0.991 0.906 2.45534692817088e-245
HLA-DRB1 1.06739496122886 0.964 0.691 1.57698834736384e-233
HLA-DQA1 1.05137342076427 0.889 0.592 2.62697974071136e-205
HLA-DQB1 1.05416893194487 0.869 0.542 1.56446935006796e-193
LTB 1.26792045211404 0.823 0.509 5.33183875969059e-190
HOPX 1.14640401940254 0.313 0.075 7.2481820031885e-186
CAPG 1.05548012718441 0.426 0.155 1.40524013342286e-157
MS4A1 0.870879536833465 0.808 0.491 6.99374162472459e-132
COTL1 0.985485336697945 0.682 0.416 3.26890815115021e-131
ARL6IP5 0.891670354292968 0.531 0.284 1.8557702023202e-105
CD99 0.874755328669385 0.481 0.28 2.24822585862373e-80
HLA-DRB5 1.06024859042027 0.554 0.369 7.2299316027442e-72
S100A4 0.949516102216384 0.45 0.337 1.5721883603198e-34
PTPRCAP 1.19802146178056 0.285 0.242 2.07312818577718e-08

Cluster 5

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGJ 2.42726834370566 0.955 0.405 0
CCL3 2.14796818946218 0.735 0.138 0
CCL4 1.69283407730557 0.658 0.131 0
IGHA1 1.52725546046667 0.995 0.849 0
SSR4 1.24082705818632 0.985 0.679 0
XBP1 1.21549349345698 0.951 0.385 0
MT-RNR2 1.17752473897542 0.951 0.415 0
LMNA 1.1684055097486 0.817 0.296 0
CST3 1.16795832082217 0.762 0.206 0
TNFRSF17 1.11168054648027 0.894 0.294 0
ANKRD28 1.10349504244139 0.762 0.221 0
CXCL14 1.09794025972713 0.719 0.15 0
APOE 1.06525043996898 0.703 0.148 0
MZB1 1.03437551072598 0.967 0.426 0
SDF2L1 1.01331242140168 0.872 0.323 2.64710988449171e-302
FKBP2 1.03508435202843 0.934 0.404 8.61408994279364e-298
SPINK2 0.983047089731138 0.505 0.119 3.01315422382261e-280
TMEM258 1.04105862843231 0.948 0.539 4.00623393337441e-278
NFKBIA 1.09071901175721 0.914 0.545 7.61040071658868e-206
DDX18 0.995261536791307 0.729 0.347 3.69511979382877e-142

Cluster 6

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGLC2 4.2496102118825 1 0.686 0
IGLC3 3.5388954116121 0.901 0.604 1.07644426990354e-300
IGLC7 1.67672223418253 0.544 0.142 2.36747011536216e-248
IGLV6-57 1.49236774524 0.361 0.121 8.56871494752713e-108
ANKRD28 0.903995696592466 0.542 0.241 1.38766454685237e-94
MZB1 0.861323281122126 0.743 0.446 1.11098782478575e-91
IGHA1 0.807956282024477 0.957 0.853 4.89871848676037e-77
HSP90B1 0.8283858277804 0.707 0.439 5.50874852913573e-62
CCL3 0.975313439828745 0.377 0.167 5.0265952102143e-58
LMNA 0.826148986929048 0.556 0.319 3.01180952963196e-51
NEAT1 0.887857570711117 0.792 0.578 8.10951010414775e-44
IGJ 0.990697974177566 0.559 0.436 5.95710395373603e-30

Cluster 7

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
EGR1 1.94734580298105 0.727 0.228 0
IER2 1.5594796375465 0.917 0.54 2.3234795662492e-239
FOS 1.62504778054477 0.937 0.708 3.07003595913588e-225
FOSB 1.14628416357706 0.92 0.581 1.22728184427347e-178
JUN 1.13597518984625 0.952 0.702 6.26289001781378e-174
NR4A1 1.18840458357601 0.708 0.329 1.50956459977159e-162
BTG2 1.19932931556595 0.858 0.552 1.58272487331689e-155
JUNB 1.12799312537507 0.975 0.797 1.50293793500644e-151
DUSP1 0.919491208942329 0.93 0.763 1.80167634846128e-107
DUSP2 1.4981935417202 0.616 0.326 2.42211622045278e-105
NR4A2 1.38384960818519 0.623 0.355 5.03410625254301e-84
ZFP36 0.874117800858897 0.831 0.594 7.63190138892749e-69
IGJ 1.31819653173397 0.594 0.434 2.18171024256469e-51
NFKBID 0.954497417289127 0.327 0.151 2.4727064403248e-51
IGHA2 0.885510067271983 0.703 0.538 3.32662303296316e-48
DNAJA1 0.987731556019389 0.61 0.399 1.49434957526899e-43
IGHA1 1.2596161579693 0.906 0.856 2.02583830827732e-41
CD69 1.34907347076495 0.643 0.603 2.60773626901635e-18
CHMP1B 1.19448615324782 0.341 0.245 7.27146360618166e-11
CD83 1.32790287403549 0.429 0.386 2.37205595146791e-09

Cluster 8

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGLL5 5.35646082655472 0.985 0.144 0
IGLL1 2.51202991955757 0.606 0.056 0
IGLC7 2.34892399955774 0.788 0.138 0
IGLV2-14 1.82052250403269 0.304 0.029 0
SSR4 1.69714308360054 0.996 0.687 1.85844959176537e-281
IGHA1 1.9738661442785 1 0.853 1.21736279366422e-259
MZB1 1.64818713566589 0.977 0.441 4.05472805728346e-252
DERL3 1.52887374896937 0.897 0.345 6.03669260529132e-250
FKBP11 1.40564059098898 0.896 0.327 4.74465579905174e-245
IGHA2 1.93947872026777 0.973 0.528 6.64866844242136e-225
FKBP2 1.39033872892029 0.924 0.419 3.19888083449405e-220
SEC11C 1.35440974211272 0.915 0.421 5.52215231066072e-208
HSP90B1 1.50371121095092 0.913 0.434 5.73761099971378e-194
IGLV1-40 1.84785865984819 0.2 0.023 5.89409546248893e-165
IGLV6-57 1.40142123639649 0.429 0.122 7.83663565941502e-129
IGLV2-8 1.66384861066201 0.169 0.023 1.51965251551568e-114
IGLV3-21 1.52711140275382 0.168 0.029 2.91607093663633e-89
IGJ 1.39063724656714 0.633 0.434 8.80872678199637e-59
IGLV3-1 1.56191326878851 0.192 0.066 9.28533221576418e-36
JCHAIN 3.55478144718525 0.367 0.311 3.49218679562612e-15

Cluster 9

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HMGB2 3.51233343447973 0.803 0.246 0
STMN1 3.50960030334085 0.775 0.141 0
TUBB 3.21479429715623 0.88 0.333 0
KIAA0101 2.61635752265166 0.668 0.036 0
GAPDH 2.43764150389359 0.988 0.805 0
HMGN1 2.40532147803212 0.961 0.613 0
PTTG1 2.39291920223073 0.674 0.088 0
RGS13 2.37873316991367 0.671 0.047 0
DUT 2.22128888010135 0.761 0.234 0
TCL1A 2.18213494101284 0.742 0.134 0
HMGA1 2.17826022516704 0.81 0.242 0
PFN1 2.12537434713581 0.988 0.794 0
ACTG1 2.07953716288919 0.991 0.797 0
ACTB 2.22836056013636 0.997 0.909 4.33466149743687e-294
HMGN2 2.98038051449322 0.903 0.541 4.12845228500669e-289
H2AFV 2.16776198284415 0.786 0.297 2.54870619193053e-279
HMGB1 2.59894274244849 0.945 0.739 2.722880968238e-273
TUBA1B 2.97722627231709 0.864 0.467 4.92917427114383e-267
H2AFZ 2.52822029552443 0.859 0.557 2.2101850660264e-226
HIST1H4C 3.00522267564898 0.614 0.32 3.36000118990649e-102

Cluster 10

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
MARCKSL1 2.74008924839173 0.942 0.316 0
ACTG1 2.44219507491093 0.998 0.798 0
RGS13 2.43750973045348 0.749 0.047 0
TCL1A 2.42535752702958 0.875 0.133 0
NEIL1 2.23370058544426 0.718 0.085 0
LRMP 2.18890669601553 0.787 0.135 0
CCDC144A 2.08838756352075 0.522 0.035 0
SERPINA9 1.98163910005879 0.55 0.019 0
HMCES 1.84347188685672 0.591 0.092 0
BASP1 1.89884856668838 0.826 0.253 9.99119593025295e-301
HMGN1 2.1375202045567 0.973 0.614 2.95657969216056e-285
CD79B 2.28433014825838 0.957 0.459 3.22590694630713e-283
LIMD2 1.99902432244834 0.939 0.44 7.75629724908882e-277
CD22 1.74083957877028 0.731 0.19 2.8562105126478e-257
LCP1 1.74667476041425 0.743 0.21 6.16826034464752e-252
ACTB 1.8154956456946 1 0.909 1.62536605134988e-242
UCP2 1.81552982352893 0.817 0.309 9.90048020700506e-213
MS4A1 1.83172858676394 0.953 0.5 9.93311528373199e-203
HLA-DMB 1.7391533806618 0.827 0.331 2.48363331135251e-201
SMIM14 1.85496260747834 0.812 0.392 3.0581853607407e-162

Cluster 11

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHG2 4.39435449798984 0.547 0.091 8.70982017802037e-291
IGHGP 3.27823389501097 0.25 0.016 3.25541392179409e-276
MZB1 1.9704272062079 0.963 0.447 2.83231472067314e-231
XBP1 1.81293378563031 0.954 0.406 4.80181386743508e-220
HSP90B1 1.83179950242274 0.919 0.439 1.6297597799665e-195
LMAN1 1.23109829396886 0.685 0.205 4.65055417348341e-178
IGHG4 4.46531565744311 0.705 0.262 4.22904530971562e-177
IGKC 2.87724221938017 0.995 0.922 2.95843545295363e-176
IGHG1 4.09160047107405 0.728 0.296 2.13330075372953e-165
FKBP11 1.36286339767389 0.854 0.334 2.28139100302147e-162
ITM2C 1.48355260790646 0.757 0.281 8.59594086539905e-162
PPIB 1.80651743329733 0.858 0.458 5.39474864397684e-161
SDF2L1 1.35128246410274 0.842 0.345 6.93694040433323e-159
IGHG3 4.24445783704195 0.738 0.329 1.16541602725006e-157
SPCS3 1.21535920058855 0.766 0.31 6.86537018524575e-144
SEC11C 1.36317155379517 0.872 0.428 1.35933673438758e-141
SUB1 1.53617296417831 0.863 0.629 3.11644027436518e-120
IGKV1-12 1.33573286234711 0.172 0.023 3.36797805734479e-98
MYDGF 1.55211745386714 0.402 0.128 8.29404582232552e-89
NEAT1 1.41036593359592 0.865 0.581 5.93886767836205e-58

Cluster 12

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHM 6.07497423919608 0.998 0.486 0
CCL3 1.23578671802024 0.357 0.173 3.69548527321943e-27
CCL4 0.934085906693097 0.321 0.162 1.62898776561341e-21
IGJ 0.95021725878952 0.497 0.441 5.19851235855153e-07

Cluster 13

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
AL928768.3 2.30499293220735 1 0.422 3.74281958128583e-291
RP5-887A10.1 1.04232438413768 0.571 0.238 9.72263200518891e-64

Cluster 14

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGLC3 3.25620013542171 1 0.611 1.24123094334271e-232
IGLC7 1.64381401465561 0.425 0.159 4.61287229187608e-48
IGLV6-57 0.824172321435519 0.24 0.132 2.96974372029905e-07

Cluster 15

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HSPA1A 3.366769084369 0.977 0.32 1.04729622700863e-283
HSPA6 2.8968037192779 0.398 0.047 5.38050852783617e-208
HSPA1B 2.52459596228512 0.898 0.321 2.64119001091802e-190
DNAJB1 2.8158252667293 0.936 0.397 1.118844728433e-187
HSPH1 2.21889113236575 0.694 0.185 1.39131896695514e-173
HSP90AA1 2.62338486341062 0.985 0.747 8.08636358658325e-161
CACYBP 2.17016672340971 0.699 0.222 8.03148080580411e-148
BAG3 1.25647166616344 0.298 0.037 1.82794458773752e-142
FKBP4 1.61605821935705 0.436 0.081 1.46893890380741e-140
HSPB1 3.25217951565696 0.791 0.346 2.90510531776409e-132
HSP90AB1 1.62186591338092 0.957 0.68 9.79016392867169e-110
HSPE1 2.24696725534384 0.888 0.562 1.19400439454616e-106
HSPD1 2.24206195332668 0.755 0.367 1.36834865239443e-102
AHSA1 1.2527281035008 0.508 0.152 3.62304695573783e-91
IER5 1.24264354537377 0.37 0.088 2.98083388371186e-83
CHORDC1 1.20206549033423 0.413 0.107 1.46178475594752e-82
DNAJA1 1.62326758794475 0.753 0.403 9.60262240144012e-79
DEDD2 1.38195990589942 0.434 0.136 2.56619187382339e-70
ZFAND2A 1.24428067050122 0.39 0.128 1.0221704098459e-55
MRPL18 1.41241387472759 0.472 0.212 1.96420503463257e-44

Cluster 16

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HLA-DQA2 2.88827324888602 0.784 0.134 2.81652833378886e-296
HLA-DRB5 2.71217429397795 0.994 0.371 1.13861095935695e-225
HLA-DRA 1.51957403090484 1 0.816 2.89241126044342e-121
IGHD 1.54577669385789 0.509 0.133 3.51267428827553e-90
CD74 1.16217423515354 1 0.96 1.05196639981035e-89
HLA-DPA1 1.29933001464275 0.985 0.738 6.18816836889087e-87
HLA-DQB1 1.37576852165395 0.93 0.56 3.40346849233345e-83
HLA-DRB1 1.06231313860207 0.98 0.707 4.97454981551205e-67
HLA-DPB1 1.11171425550068 0.968 0.725 3.09492373736976e-66
BTG1 1.03081094312085 0.988 0.892 8.06229638987534e-65
CXCR4 1.10937663253161 0.965 0.678 5.20991689236672e-58
HLA-DMA 1.21742236496268 0.784 0.49 5.24103680799545e-53
HLA-DQA1 1.13880247183311 0.88 0.61 2.40013244765968e-52
LAPTM5 1.07576564295753 0.88 0.627 2.81595438755948e-49
PHACTR1 1.12386207367035 0.439 0.184 9.3582013809045e-36
SARAF 1.21392537553512 0.596 0.334 1.31059646550242e-34
CD72 0.981927860941594 0.333 0.121 4.40506969775615e-31
CD83 1.06118322503405 0.649 0.383 1.57326868843079e-30
CREM 1.15192297802742 0.512 0.3 3.97685825561876e-21
LY9 0.970227312720828 0.442 0.257 2.48195952506625e-16

Cluster 17

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHG2 5.31409682070497 0.897 0.092 0
IGHGP 4.30039886492274 0.559 0.015 0
IGHG1 5.66625995887964 0.983 0.299 6.00234324007762e-243
IGHG4 4.94698110219705 0.955 0.265 6.53484146219843e-240
IGHG3 5.58319667648052 0.993 0.331 3.17059448658041e-235
IGLC7 2.25388917205277 0.707 0.156 1.15091670089511e-144
MYDGF 1.86393061130477 0.614 0.129 3.80265403779637e-140
MZB1 2.19997319663866 0.983 0.455 1.79522661363043e-133
XBP1 1.97956959794966 0.969 0.415 1.2464497316502e-123
FKBP11 1.82790747242575 0.924 0.341 1.76034969173006e-120
IGLL5 3.97391925458365 0.645 0.171 4.13816836975115e-115
IGLC2 3.95666142866195 0.99 0.7 8.74567240894911e-115
IGLC3 3.09250041453404 0.983 0.615 6.92810286256675e-109
HSP90B1 1.73243656383935 0.966 0.446 1.46704699978872e-106
PRDX4 1.67665701105899 0.845 0.309 2.20336011513178e-104
IGLV6-57 2.61597783721131 0.531 0.128 9.29352014489006e-95
DERL3 1.71625242514733 0.897 0.36 5.92579479347103e-93
IGLV1-51 3.08657260738475 0.207 0.023 1.9135733985745e-80
IGLV3-21 2.02424392620283 0.166 0.032 5.02171823054164e-31
IGLV2-23 2.14328154518304 0.134 0.027 8.67468636623694e-24

Cluster 18

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
CD3E 1.87039499840996 0.69 0.018 0
CD2 1.86385706252177 0.675 0.033 0
FYB 1.79040093297581 0.675 0.032 0
CD3G 1.37218139135589 0.556 0.013 0
IL7R 1.94589036274448 0.54 0.022 7.65405742387953e-301
CD3D 2.4683420605671 0.897 0.089 3.72991460712307e-230
CD7 1.90571744713958 0.619 0.041 3.13489043658798e-218
GIMAP7 1.02839562202744 0.397 0.016 2.83402014774046e-211
IL32 2.68231800205667 0.754 0.096 3.83217699931357e-144
TRAC 2.10081674503777 0.865 0.17 1.37017353940225e-103
TRBC1 1.59355220080298 0.214 0.01 9.55569911073656e-103
TNFAIP3 1.45078913343237 0.675 0.129 1.10569487390731e-73
ANXA1 2.10307898999391 0.468 0.071 1.66285191746957e-65
TRBC2 1.64626725635072 0.786 0.208 1.2995641399185e-58
KLRB1 2.26430650393556 0.381 0.065 3.16176479998516e-46
HCST 1.1964474781201 0.706 0.195 7.3491153824209e-46
GZMK 1.26460109178382 0.151 0.011 1.11390089133479e-42
LEPROTL1 1.03049600190134 0.54 0.132 1.87785746427521e-38
S100A4 1.65655867610182 0.69 0.343 1.4149067667347e-18
CCL5 1.68829097063693 0.302 0.097 5.50417144119652e-12

First annotations

Clusters Annotation Clusters Annotation
0 ? 10 antigen activated?
1 Naive 11 Plasma cells secreting IgG+
2 ? 12 Plasma cells secreting IgM+
3 Plasma secreting IgA+ 13 ?
4 ? 14 ?
5 Plasma secreting IgA+ 15 ? (overexpression of heat-shock proteins)
6 IgA+ 16 Naive
7 Antigen-activated Bcell IgA+ 17 Plasma cells secreting IgG+
8 Plasma secreting IgA+ 18 ??
9 antigen activated? - -

Hierarchical Clustering of Bcells Clusters

Merge some clusters together and find new markers

Considering previous results, the following clusters were merged together:

Following merge, markers were calculated for the new clusters:

Cluster 0_2

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
RP5-887A10.1 1.34385115490503 0.479 0.177 0
GPR183 1.16007407822242 0.588 0.293 0
RPS29 0.87846568428133 0.993 0.945 0
TOMM7 0.818464712319199 0.914 0.802 0
RPL38 0.809898977514249 0.983 0.919 0
RPS12 0.809033557586074 0.999 0.983 0
ARHGAP24 0.879341504328434 0.438 0.18 4.62650544712939e-268

Cluster 1_16

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHD 2.34537110387508 0.619 0.062 0
TCL1A 1.69758261514395 0.54 0.096 0
FCER2 1.6267393699699 0.466 0.083 0
BTG1 1.26839453709142 0.986 0.878 0
CXCR4 1.22541001010976 0.964 0.638 0
CD72 1.21638429763728 0.366 0.086 0
HLA-DRB1 1.18339620799049 0.932 0.676 0
LAPTM5 1.17464722233147 0.89 0.589 0
HLA-DRA 1.12413394542217 0.998 0.79 0
CD52 1.1208173270258 0.955 0.694 0
HLA-DPA1 1.10719636147749 0.956 0.708 0
HLA-DQA1 1.06673322433735 0.847 0.577 0
HLA-DPB1 1.03668309854372 0.958 0.692 0
HLA-DQB1 1.06556205978242 0.811 0.527 4.08777324781938e-281
YBX3 1.23638289201699 0.298 0.077 1.82580358887704e-267
HLA-DMA 1.03103348439754 0.723 0.459 5.37021880364412e-261
SARAF 1.15331383577432 0.536 0.307 3.48539435775643e-170
HLA-DRB5 1.31026945933426 0.565 0.353 4.58129410373894e-167
CHMP1B 1.12688600614479 0.385 0.228 9.62046001397265e-95
HLA-DQA2 1.15237936919729 0.273 0.125 4.30813332812843e-92

Cluster 3_5_6_8

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHA2 3.4346700644468 0.935 0.408 0
IGHA1 3.1657096013776 0.989 0.813 0
IGLL5 2.66672939543959 0.414 0.095 0
IGJ 2.58991562775378 0.724 0.342 0
IGKC 2.32717323409743 0.961 0.911 0
AC096579.7 2.32273732600444 0.417 0.083 0
IGLC2 2.04974723506305 0.859 0.649 0
SSR4 1.97079506462838 0.955 0.608 0
CCL3 1.88310400635241 0.401 0.1 0
MZB1 1.85548507189336 0.913 0.302 0
DERL3 1.6126125685226 0.788 0.218 0
TNFRSF17 1.59226881259828 0.754 0.186 0
XBP1 1.56586069222735 0.832 0.278 0
FKBP2 1.55991092845702 0.834 0.3 0
FKBP11 1.54096169229181 0.788 0.195 0
SEC11C 1.52537747973113 0.823 0.306 0
HSPA1B 1.52116788196665 0.638 0.225 0
DNAJB9 1.4831609904652 0.746 0.243 0
HERPUD1 1.47713815979417 0.945 0.687 0
ANKRD28 1.43804164135754 0.582 0.142 0

Cluster 4_13

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HLA-DPB1 1.19656328507929 0.971 0.702 0
HLA-DPA1 1.17926410676914 0.97 0.717 0
HLA-DRA 1.09899110387575 0.997 0.799 0
HLA-A 1.06308469559813 0.98 0.883 0
CD74 1.04515432868497 1 0.956 0
TMSB4X 0.83783304970108 1 0.992 0
ACTB 0.835791908192409 0.99 0.904 2.05119359997549e-285
HLA-DRB1 1.00847078563524 0.956 0.684 3.7159255627956e-284
HLA-DQA1 0.981670404636775 0.88 0.585 2.5758611453842e-244
HLA-DQB1 0.99982387840055 0.865 0.533 6.95764712765883e-244
LTB 1.21100639711323 0.826 0.499 1.44639280628271e-241
CD52 0.828915293832535 0.94 0.707 9.18244271228736e-193
CAPG 1.00855129835576 0.402 0.149 1.75896108443946e-180
HOPX 0.977633874748074 0.265 0.073 6.85501620850976e-158
COTL1 0.910812153837835 0.666 0.41 2.7847112386827e-150
ARL6IP5 0.864868018743337 0.516 0.278 3.39789374494364e-127
CD82 0.813838537738884 0.364 0.148 2.45129521499358e-125
HLA-DRB5 0.910790632492023 0.513 0.368 1.42187756172243e-61
S100A4 0.860703526828224 0.438 0.335 5.25463165713307e-39
AL928768.3 0.862927586086103 0.491 0.431 1.3236795264431e-26

Cluster 9_10

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
HMGB2 2.92952537169356 0.686 0.235 0
STMN1 2.92238996770972 0.58 0.133 0
RGS13 2.67405162070697 0.707 0.021 0
MARCKSL1 2.58761345936642 0.874 0.295 0
HMGN2 2.56483693461446 0.858 0.531 0
TCL1A 2.54316558006607 0.804 0.107 0
HMGN1 2.51967252650735 0.967 0.6 0
ACTG1 2.49233603781354 0.995 0.79 0
GAPDH 2.31122483742834 0.988 0.799 0
ACTB 2.2319998734064 0.998 0.906 0
LRMP 2.16101657724544 0.728 0.112 0
HMGB1 2.15665752499151 0.941 0.732 0
KIAA0101 2.05437973454933 0.457 0.03 0
HMGA1 2.02334920838714 0.771 0.224 0
PFN1 1.98504313250158 0.985 0.787 0
PTTG1 1.96114217957876 0.552 0.076 0
TUBB 2.51931732619148 0.699 0.327 1.30118165133735e-284
H2AFZ 1.9380078037232 0.796 0.55 1.57651365928373e-195
TUBA1B 2.21933887039883 0.666 0.468 1.08057408031387e-124
HIST1H4C 2.27304930241359 0.498 0.319 2.18817875859149e-61

Cluster 11_17

Gene Average Log2FC % Expressed in cluster % Expressed others p-value adjusted
IGHG3 6.58946866929302 0.824 0.317 0
IGHG1 6.48364357447739 0.814 0.284 0
IGHG2 6.38783455650974 0.665 0.077 0
IGHG4 5.98026342256049 0.789 0.25 0
IGHGP 4.24114011163245 0.354 0.007 0
MZB1 2.16615918215015 0.97 0.437 0
XBP1 1.96600273595554 0.959 0.397 0
HSP90B1 1.88139009770943 0.935 0.43 0
FKBP11 1.6078257326899 0.878 0.324 1.85020864825481e-289
ITM2C 1.60113769928362 0.776 0.271 3.69576696435799e-269
PPIB 1.7542724222688 0.867 0.45 1.16893149741511e-244
SEC11C 1.41830824137654 0.886 0.419 4.85480285846436e-234
MYDGF 1.74412574921146 0.474 0.119 2.09691565298866e-219
SUB1 1.41161685793752 0.879 0.624 4.70904176431471e-159
NEAT1 1.43043497427383 0.877 0.575 5.60162450599924e-113
IGKC 2.29274627357827 0.983 0.921 2.40377956289766e-69
IGLV6-57 1.49036739354878 0.326 0.125 1.20907014335966e-61
IGLL5 2.4318009651225 0.368 0.169 5.65050625562366e-49
IGLC3 1.97897254079235 0.745 0.615 3.70465460281764e-19
IGLC2 2.66808462629656 0.807 0.699 1.90274127994769e-17

Second annotations

Clusters Annotation Clusters Annotation
0_2 Memory? 11_17 Plasma cells secreting IgG+
1_16 Naive 12 ?? (IgM+)
3_5_6_8 Plasma cells secreting IgA+ 14 ??
4_13 Memory? 15 ? (overexpression of heat-shock proteins)
7 ?? (IgA+) 18 ??
9_10 Plasmablast? - -