Bcells

Bcells Clusters

Gene Expression

Kappa vs Lambda Expression

Igs Expression

Other Genes’ Expression

Clusters’ Gene Markers

Cluster 0

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
GPR183	1.3872860905509	0.643	0.321	1.50093013586677e-293
CXCR4	0.897002084782664	0.946	0.646	2.79465114456748e-269
NR4A2	1.01120472991501	0.58	0.339	1.57297953329415e-168
ARHGAP24	0.951281223665877	0.454	0.209	9.03405876935521e-167
CD83	0.80962903633932	0.596	0.359	2.31508248081161e-135
REL	0.843537344418049	0.52	0.301	8.6653603382112e-117
LY9	0.82689440420669	0.401	0.241	7.02464835412563e-77
CREM	0.943289403623355	0.372	0.295	6.09109551075867e-27

Cluster 1

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHD	2.26937422631391	0.636	0.072	0
TCL1A	1.7630542485369	0.58	0.1	0
FCER2	1.64147629158705	0.492	0.088	0
BTG1	1.22546167629801	0.986	0.881	0
CD72	1.17805125124466	0.372	0.091	0
CXCR4	1.16152267294715	0.964	0.645	0
HLA-DRB1	1.12500543899733	0.924	0.682	0
CD52	1.11943294122547	0.957	0.699	0
LAPTM5	1.11238598612084	0.892	0.596	0
HLA-DPA1	0.982993408017732	0.951	0.714	0
HLA-DPB1	0.949419931075115	0.957	0.698	0
HLA-DRA	0.940153958621598	0.998	0.795	0
YBX3	1.2467485659192	0.31	0.08	4.48455805454258e-253
HLA-DQA1	0.977022935929604	0.841	0.584	9.28421960879641e-248
IL4R	1.06495586857944	0.261	0.065	4.80146387161673e-208
SELL	0.991289417372965	0.416	0.18	2.73692488119944e-160
RHOH	0.970306103581982	0.613	0.427	3.30046781677669e-144
STAG3	1.01814473289099	0.291	0.11	2.55554256341291e-130
SARAF	1.05688550927337	0.527	0.313	5.60938809293103e-128
CHMP1B	1.13859186116654	0.388	0.231	2.96314808802244e-83

Cluster 2

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
RP5-887A10.1	1.49968785886481	0.624	0.203	0
RPS29	0.939276659481525	0.998	0.951	0
RPS12	0.928704871719422	1	0.985	0
RPL38	0.897563425097028	0.995	0.927	0
RPL39	0.869893889866293	0.996	0.901	0
RPS21	0.859690733291504	0.994	0.924	0
RPL31	0.824896983932141	1	0.974	0
RPL30	0.82108216047948	1	0.969	0
RPLP2	0.819383349060717	1	0.992	0
RPS27	0.812621288758966	1	0.991	0
TOMM7	0.817238749661796	0.959	0.813	3.70253350907267e-236

Cluster 3

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
AC096579.7	3.40185614921032	0.572	0.128	0
IGKC	3.3591239326899	0.996	0.916	0
IGHA2	3.16260146988569	0.97	0.502	0
IGHA1	2.30823974363442	0.998	0.844	0
HSPA1B	2.02938341373645	0.703	0.294	0
HERPUD1	1.45159797626684	0.941	0.735	0
DERL3	1.4436639512233	0.767	0.326	0
SSR4	1.37709447993418	0.969	0.671	0
MZB1	1.35426809163824	0.946	0.412	0
FKBP11	1.33040682452385	0.761	0.307	0
JUN	1.6746957451251	0.905	0.695	3.55632562274781e-291
TIMP1	1.31134621758947	0.587	0.239	5.13874886366238e-258
AL928768.3	1.44027172669107	0.774	0.401	5.08508130151852e-246
DNAJB9	1.34276272655493	0.69	0.342	7.97051016225126e-244
HSPA1A	1.72537412715778	0.61	0.305	3.91131484789896e-209
CITED2	1.3161938486866	0.532	0.249	5.69973293932676e-171
HSPA5	1.32116832808557	0.636	0.377	2.4446798274215e-158
IER2	1.50598642084791	0.743	0.542	1.10956700929047e-141
RGCC	1.38600252789561	0.488	0.281	1.85332678531166e-87
JCHAIN	2.44362074120798	0.301	0.315	0.0024070944722449

Cluster 4

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HLA-DPB1	1.2950921450342	0.983	0.709	0
HLA-DPA1	1.25308704746769	0.976	0.723	0
HLA-DRA	1.14090035178467	0.999	0.805	0
CD74	1.09885364846992	1	0.957	0
TMSB4X	0.911356013287482	1	0.992	9.33778827268325e-277
HLA-A	1.08157550050729	0.981	0.886	1.1981664192463e-263
ACTB	0.925010927903783	0.991	0.906	2.45534692817088e-245
HLA-DRB1	1.06739496122886	0.964	0.691	1.57698834736384e-233
HLA-DQA1	1.05137342076427	0.889	0.592	2.62697974071136e-205
HLA-DQB1	1.05416893194487	0.869	0.542	1.56446935006796e-193
LTB	1.26792045211404	0.823	0.509	5.33183875969059e-190
HOPX	1.14640401940254	0.313	0.075	7.2481820031885e-186
CAPG	1.05548012718441	0.426	0.155	1.40524013342286e-157
MS4A1	0.870879536833465	0.808	0.491	6.99374162472459e-132
COTL1	0.985485336697945	0.682	0.416	3.26890815115021e-131
ARL6IP5	0.891670354292968	0.531	0.284	1.8557702023202e-105
CD99	0.874755328669385	0.481	0.28	2.24822585862373e-80
HLA-DRB5	1.06024859042027	0.554	0.369	7.2299316027442e-72
S100A4	0.949516102216384	0.45	0.337	1.5721883603198e-34
PTPRCAP	1.19802146178056	0.285	0.242	2.07312818577718e-08

Cluster 5

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGJ	2.42726834370566	0.955	0.405	0
CCL3	2.14796818946218	0.735	0.138	0
CCL4	1.69283407730557	0.658	0.131	0
IGHA1	1.52725546046667	0.995	0.849	0
SSR4	1.24082705818632	0.985	0.679	0
XBP1	1.21549349345698	0.951	0.385	0
MT-RNR2	1.17752473897542	0.951	0.415	0
LMNA	1.1684055097486	0.817	0.296	0
CST3	1.16795832082217	0.762	0.206	0
TNFRSF17	1.11168054648027	0.894	0.294	0
ANKRD28	1.10349504244139	0.762	0.221	0
CXCL14	1.09794025972713	0.719	0.15	0
APOE	1.06525043996898	0.703	0.148	0
MZB1	1.03437551072598	0.967	0.426	0
SDF2L1	1.01331242140168	0.872	0.323	2.64710988449171e-302
FKBP2	1.03508435202843	0.934	0.404	8.61408994279364e-298
SPINK2	0.983047089731138	0.505	0.119	3.01315422382261e-280
TMEM258	1.04105862843231	0.948	0.539	4.00623393337441e-278
NFKBIA	1.09071901175721	0.914	0.545	7.61040071658868e-206
DDX18	0.995261536791307	0.729	0.347	3.69511979382877e-142

Cluster 6

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGLC2	4.2496102118825	1	0.686	0
IGLC3	3.5388954116121	0.901	0.604	1.07644426990354e-300
IGLC7	1.67672223418253	0.544	0.142	2.36747011536216e-248
IGLV6-57	1.49236774524	0.361	0.121	8.56871494752713e-108
ANKRD28	0.903995696592466	0.542	0.241	1.38766454685237e-94
MZB1	0.861323281122126	0.743	0.446	1.11098782478575e-91
IGHA1	0.807956282024477	0.957	0.853	4.89871848676037e-77
HSP90B1	0.8283858277804	0.707	0.439	5.50874852913573e-62
CCL3	0.975313439828745	0.377	0.167	5.0265952102143e-58
LMNA	0.826148986929048	0.556	0.319	3.01180952963196e-51
NEAT1	0.887857570711117	0.792	0.578	8.10951010414775e-44
IGJ	0.990697974177566	0.559	0.436	5.95710395373603e-30

Cluster 7

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
EGR1	1.94734580298105	0.727	0.228	0
IER2	1.5594796375465	0.917	0.54	2.3234795662492e-239
FOS	1.62504778054477	0.937	0.708	3.07003595913588e-225
FOSB	1.14628416357706	0.92	0.581	1.22728184427347e-178
JUN	1.13597518984625	0.952	0.702	6.26289001781378e-174
NR4A1	1.18840458357601	0.708	0.329	1.50956459977159e-162
BTG2	1.19932931556595	0.858	0.552	1.58272487331689e-155
JUNB	1.12799312537507	0.975	0.797	1.50293793500644e-151
DUSP1	0.919491208942329	0.93	0.763	1.80167634846128e-107
DUSP2	1.4981935417202	0.616	0.326	2.42211622045278e-105
NR4A2	1.38384960818519	0.623	0.355	5.03410625254301e-84
ZFP36	0.874117800858897	0.831	0.594	7.63190138892749e-69
IGJ	1.31819653173397	0.594	0.434	2.18171024256469e-51
NFKBID	0.954497417289127	0.327	0.151	2.4727064403248e-51
IGHA2	0.885510067271983	0.703	0.538	3.32662303296316e-48
DNAJA1	0.987731556019389	0.61	0.399	1.49434957526899e-43
IGHA1	1.2596161579693	0.906	0.856	2.02583830827732e-41
CD69	1.34907347076495	0.643	0.603	2.60773626901635e-18
CHMP1B	1.19448615324782	0.341	0.245	7.27146360618166e-11
CD83	1.32790287403549	0.429	0.386	2.37205595146791e-09

Cluster 8

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGLL5	5.35646082655472	0.985	0.144	0
IGLL1	2.51202991955757	0.606	0.056	0
IGLC7	2.34892399955774	0.788	0.138	0
IGLV2-14	1.82052250403269	0.304	0.029	0
SSR4	1.69714308360054	0.996	0.687	1.85844959176537e-281
IGHA1	1.9738661442785	1	0.853	1.21736279366422e-259
MZB1	1.64818713566589	0.977	0.441	4.05472805728346e-252
DERL3	1.52887374896937	0.897	0.345	6.03669260529132e-250
FKBP11	1.40564059098898	0.896	0.327	4.74465579905174e-245
IGHA2	1.93947872026777	0.973	0.528	6.64866844242136e-225
FKBP2	1.39033872892029	0.924	0.419	3.19888083449405e-220
SEC11C	1.35440974211272	0.915	0.421	5.52215231066072e-208
HSP90B1	1.50371121095092	0.913	0.434	5.73761099971378e-194
IGLV1-40	1.84785865984819	0.2	0.023	5.89409546248893e-165
IGLV6-57	1.40142123639649	0.429	0.122	7.83663565941502e-129
IGLV2-8	1.66384861066201	0.169	0.023	1.51965251551568e-114
IGLV3-21	1.52711140275382	0.168	0.029	2.91607093663633e-89
IGJ	1.39063724656714	0.633	0.434	8.80872678199637e-59
IGLV3-1	1.56191326878851	0.192	0.066	9.28533221576418e-36
JCHAIN	3.55478144718525	0.367	0.311	3.49218679562612e-15

Cluster 9

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HMGB2	3.51233343447973	0.803	0.246	0
STMN1	3.50960030334085	0.775	0.141	0
TUBB	3.21479429715623	0.88	0.333	0
KIAA0101	2.61635752265166	0.668	0.036	0
GAPDH	2.43764150389359	0.988	0.805	0
HMGN1	2.40532147803212	0.961	0.613	0
PTTG1	2.39291920223073	0.674	0.088	0
RGS13	2.37873316991367	0.671	0.047	0
DUT	2.22128888010135	0.761	0.234	0
TCL1A	2.18213494101284	0.742	0.134	0
HMGA1	2.17826022516704	0.81	0.242	0
PFN1	2.12537434713581	0.988	0.794	0
ACTG1	2.07953716288919	0.991	0.797	0
ACTB	2.22836056013636	0.997	0.909	4.33466149743687e-294
HMGN2	2.98038051449322	0.903	0.541	4.12845228500669e-289
H2AFV	2.16776198284415	0.786	0.297	2.54870619193053e-279
HMGB1	2.59894274244849	0.945	0.739	2.722880968238e-273
TUBA1B	2.97722627231709	0.864	0.467	4.92917427114383e-267
H2AFZ	2.52822029552443	0.859	0.557	2.2101850660264e-226
HIST1H4C	3.00522267564898	0.614	0.32	3.36000118990649e-102

Cluster 10

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
MARCKSL1	2.74008924839173	0.942	0.316	0
ACTG1	2.44219507491093	0.998	0.798	0
RGS13	2.43750973045348	0.749	0.047	0
TCL1A	2.42535752702958	0.875	0.133	0
NEIL1	2.23370058544426	0.718	0.085	0
LRMP	2.18890669601553	0.787	0.135	0
CCDC144A	2.08838756352075	0.522	0.035	0
SERPINA9	1.98163910005879	0.55	0.019	0
HMCES	1.84347188685672	0.591	0.092	0
BASP1	1.89884856668838	0.826	0.253	9.99119593025295e-301
HMGN1	2.1375202045567	0.973	0.614	2.95657969216056e-285
CD79B	2.28433014825838	0.957	0.459	3.22590694630713e-283
LIMD2	1.99902432244834	0.939	0.44	7.75629724908882e-277
CD22	1.74083957877028	0.731	0.19	2.8562105126478e-257
LCP1	1.74667476041425	0.743	0.21	6.16826034464752e-252
ACTB	1.8154956456946	1	0.909	1.62536605134988e-242
UCP2	1.81552982352893	0.817	0.309	9.90048020700506e-213
MS4A1	1.83172858676394	0.953	0.5	9.93311528373199e-203
HLA-DMB	1.7391533806618	0.827	0.331	2.48363331135251e-201
SMIM14	1.85496260747834	0.812	0.392	3.0581853607407e-162

Cluster 11

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHG2	4.39435449798984	0.547	0.091	8.70982017802037e-291
IGHGP	3.27823389501097	0.25	0.016	3.25541392179409e-276
MZB1	1.9704272062079	0.963	0.447	2.83231472067314e-231
XBP1	1.81293378563031	0.954	0.406	4.80181386743508e-220
HSP90B1	1.83179950242274	0.919	0.439	1.6297597799665e-195
LMAN1	1.23109829396886	0.685	0.205	4.65055417348341e-178
IGHG4	4.46531565744311	0.705	0.262	4.22904530971562e-177
IGKC	2.87724221938017	0.995	0.922	2.95843545295363e-176
IGHG1	4.09160047107405	0.728	0.296	2.13330075372953e-165
FKBP11	1.36286339767389	0.854	0.334	2.28139100302147e-162
ITM2C	1.48355260790646	0.757	0.281	8.59594086539905e-162
PPIB	1.80651743329733	0.858	0.458	5.39474864397684e-161
SDF2L1	1.35128246410274	0.842	0.345	6.93694040433323e-159
IGHG3	4.24445783704195	0.738	0.329	1.16541602725006e-157
SPCS3	1.21535920058855	0.766	0.31	6.86537018524575e-144
SEC11C	1.36317155379517	0.872	0.428	1.35933673438758e-141
SUB1	1.53617296417831	0.863	0.629	3.11644027436518e-120
IGKV1-12	1.33573286234711	0.172	0.023	3.36797805734479e-98
MYDGF	1.55211745386714	0.402	0.128	8.29404582232552e-89
NEAT1	1.41036593359592	0.865	0.581	5.93886767836205e-58

Cluster 12

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHM	6.07497423919608	0.998	0.486	0
CCL3	1.23578671802024	0.357	0.173	3.69548527321943e-27
CCL4	0.934085906693097	0.321	0.162	1.62898776561341e-21
IGJ	0.95021725878952	0.497	0.441	5.19851235855153e-07

Cluster 13

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
AL928768.3	2.30499293220735	1	0.422	3.74281958128583e-291
RP5-887A10.1	1.04232438413768	0.571	0.238	9.72263200518891e-64

Cluster 14

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGLC3	3.25620013542171	1	0.611	1.24123094334271e-232
IGLC7	1.64381401465561	0.425	0.159	4.61287229187608e-48
IGLV6-57	0.824172321435519	0.24	0.132	2.96974372029905e-07

Cluster 15

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HSPA1A	3.366769084369	0.977	0.32	1.04729622700863e-283
HSPA6	2.8968037192779	0.398	0.047	5.38050852783617e-208
HSPA1B	2.52459596228512	0.898	0.321	2.64119001091802e-190
DNAJB1	2.8158252667293	0.936	0.397	1.118844728433e-187
HSPH1	2.21889113236575	0.694	0.185	1.39131896695514e-173
HSP90AA1	2.62338486341062	0.985	0.747	8.08636358658325e-161
CACYBP	2.17016672340971	0.699	0.222	8.03148080580411e-148
BAG3	1.25647166616344	0.298	0.037	1.82794458773752e-142
FKBP4	1.61605821935705	0.436	0.081	1.46893890380741e-140
HSPB1	3.25217951565696	0.791	0.346	2.90510531776409e-132
HSP90AB1	1.62186591338092	0.957	0.68	9.79016392867169e-110
HSPE1	2.24696725534384	0.888	0.562	1.19400439454616e-106
HSPD1	2.24206195332668	0.755	0.367	1.36834865239443e-102
AHSA1	1.2527281035008	0.508	0.152	3.62304695573783e-91
IER5	1.24264354537377	0.37	0.088	2.98083388371186e-83
CHORDC1	1.20206549033423	0.413	0.107	1.46178475594752e-82
DNAJA1	1.62326758794475	0.753	0.403	9.60262240144012e-79
DEDD2	1.38195990589942	0.434	0.136	2.56619187382339e-70
ZFAND2A	1.24428067050122	0.39	0.128	1.0221704098459e-55
MRPL18	1.41241387472759	0.472	0.212	1.96420503463257e-44

Cluster 16

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HLA-DQA2	2.88827324888602	0.784	0.134	2.81652833378886e-296
HLA-DRB5	2.71217429397795	0.994	0.371	1.13861095935695e-225
HLA-DRA	1.51957403090484	1	0.816	2.89241126044342e-121
IGHD	1.54577669385789	0.509	0.133	3.51267428827553e-90
CD74	1.16217423515354	1	0.96	1.05196639981035e-89
HLA-DPA1	1.29933001464275	0.985	0.738	6.18816836889087e-87
HLA-DQB1	1.37576852165395	0.93	0.56	3.40346849233345e-83
HLA-DRB1	1.06231313860207	0.98	0.707	4.97454981551205e-67
HLA-DPB1	1.11171425550068	0.968	0.725	3.09492373736976e-66
BTG1	1.03081094312085	0.988	0.892	8.06229638987534e-65
CXCR4	1.10937663253161	0.965	0.678	5.20991689236672e-58
HLA-DMA	1.21742236496268	0.784	0.49	5.24103680799545e-53
HLA-DQA1	1.13880247183311	0.88	0.61	2.40013244765968e-52
LAPTM5	1.07576564295753	0.88	0.627	2.81595438755948e-49
PHACTR1	1.12386207367035	0.439	0.184	9.3582013809045e-36
SARAF	1.21392537553512	0.596	0.334	1.31059646550242e-34
CD72	0.981927860941594	0.333	0.121	4.40506969775615e-31
CD83	1.06118322503405	0.649	0.383	1.57326868843079e-30
CREM	1.15192297802742	0.512	0.3	3.97685825561876e-21
LY9	0.970227312720828	0.442	0.257	2.48195952506625e-16

Cluster 17

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHG2	5.31409682070497	0.897	0.092	0
IGHGP	4.30039886492274	0.559	0.015	0
IGHG1	5.66625995887964	0.983	0.299	6.00234324007762e-243
IGHG4	4.94698110219705	0.955	0.265	6.53484146219843e-240
IGHG3	5.58319667648052	0.993	0.331	3.17059448658041e-235
IGLC7	2.25388917205277	0.707	0.156	1.15091670089511e-144
MYDGF	1.86393061130477	0.614	0.129	3.80265403779637e-140
MZB1	2.19997319663866	0.983	0.455	1.79522661363043e-133
XBP1	1.97956959794966	0.969	0.415	1.2464497316502e-123
FKBP11	1.82790747242575	0.924	0.341	1.76034969173006e-120
IGLL5	3.97391925458365	0.645	0.171	4.13816836975115e-115
IGLC2	3.95666142866195	0.99	0.7	8.74567240894911e-115
IGLC3	3.09250041453404	0.983	0.615	6.92810286256675e-109
HSP90B1	1.73243656383935	0.966	0.446	1.46704699978872e-106
PRDX4	1.67665701105899	0.845	0.309	2.20336011513178e-104
IGLV6-57	2.61597783721131	0.531	0.128	9.29352014489006e-95
DERL3	1.71625242514733	0.897	0.36	5.92579479347103e-93
IGLV1-51	3.08657260738475	0.207	0.023	1.9135733985745e-80
IGLV3-21	2.02424392620283	0.166	0.032	5.02171823054164e-31
IGLV2-23	2.14328154518304	0.134	0.027	8.67468636623694e-24

Cluster 18

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
CD3E	1.87039499840996	0.69	0.018	0
CD2	1.86385706252177	0.675	0.033	0
FYB	1.79040093297581	0.675	0.032	0
CD3G	1.37218139135589	0.556	0.013	0
IL7R	1.94589036274448	0.54	0.022	7.65405742387953e-301
CD3D	2.4683420605671	0.897	0.089	3.72991460712307e-230
CD7	1.90571744713958	0.619	0.041	3.13489043658798e-218
GIMAP7	1.02839562202744	0.397	0.016	2.83402014774046e-211
IL32	2.68231800205667	0.754	0.096	3.83217699931357e-144
TRAC	2.10081674503777	0.865	0.17	1.37017353940225e-103
TRBC1	1.59355220080298	0.214	0.01	9.55569911073656e-103
TNFAIP3	1.45078913343237	0.675	0.129	1.10569487390731e-73
ANXA1	2.10307898999391	0.468	0.071	1.66285191746957e-65
TRBC2	1.64626725635072	0.786	0.208	1.2995641399185e-58
KLRB1	2.26430650393556	0.381	0.065	3.16176479998516e-46
HCST	1.1964474781201	0.706	0.195	7.3491153824209e-46
GZMK	1.26460109178382	0.151	0.011	1.11390089133479e-42
LEPROTL1	1.03049600190134	0.54	0.132	1.87785746427521e-38
S100A4	1.65655867610182	0.69	0.343	1.4149067667347e-18
CCL5	1.68829097063693	0.302	0.097	5.50417144119652e-12

First annotations

Clusters	Annotation	Clusters	Annotation
0	?	10	antigen activated?
1	Naive	11	Plasma cells secreting IgG+
2	?	12	Plasma cells secreting IgM+
3	Plasma secreting IgA+	13	?
4	?	14	?
5	Plasma secreting IgA+	15	? (overexpression of heat-shock proteins)
6	IgA+	16	Naive
7	Antigen-activated Bcell IgA+	17	Plasma cells secreting IgG+
8	Plasma secreting IgA+	18	??
9	antigen activated?	-	-

Hierarchical Clustering of Bcells Clusters

Merge some clusters together and find new markers

Considering previous results, the following clusters were merged together:

• 11 + 17;

• 3 + 5 + 6 + 8;

• 9 + 10;

• 1 + 16;

• 0 + 2;

• 4 + 13.

Following merge, markers were calculated for the new clusters:

Cluster 0_2

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
RP5-887A10.1	1.34385115490503	0.479	0.177	0
GPR183	1.16007407822242	0.588	0.293	0
RPS29	0.87846568428133	0.993	0.945	0
TOMM7	0.818464712319199	0.914	0.802	0
RPL38	0.809898977514249	0.983	0.919	0
RPS12	0.809033557586074	0.999	0.983	0
ARHGAP24	0.879341504328434	0.438	0.18	4.62650544712939e-268

Cluster 1_16

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHD	2.34537110387508	0.619	0.062	0
TCL1A	1.69758261514395	0.54	0.096	0
FCER2	1.6267393699699	0.466	0.083	0
BTG1	1.26839453709142	0.986	0.878	0
CXCR4	1.22541001010976	0.964	0.638	0
CD72	1.21638429763728	0.366	0.086	0
HLA-DRB1	1.18339620799049	0.932	0.676	0
LAPTM5	1.17464722233147	0.89	0.589	0
HLA-DRA	1.12413394542217	0.998	0.79	0
CD52	1.1208173270258	0.955	0.694	0
HLA-DPA1	1.10719636147749	0.956	0.708	0
HLA-DQA1	1.06673322433735	0.847	0.577	0
HLA-DPB1	1.03668309854372	0.958	0.692	0
HLA-DQB1	1.06556205978242	0.811	0.527	4.08777324781938e-281
YBX3	1.23638289201699	0.298	0.077	1.82580358887704e-267
HLA-DMA	1.03103348439754	0.723	0.459	5.37021880364412e-261
SARAF	1.15331383577432	0.536	0.307	3.48539435775643e-170
HLA-DRB5	1.31026945933426	0.565	0.353	4.58129410373894e-167
CHMP1B	1.12688600614479	0.385	0.228	9.62046001397265e-95
HLA-DQA2	1.15237936919729	0.273	0.125	4.30813332812843e-92

Cluster 3_5_6_8

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHA2	3.4346700644468	0.935	0.408	0
IGHA1	3.1657096013776	0.989	0.813	0
IGLL5	2.66672939543959	0.414	0.095	0
IGJ	2.58991562775378	0.724	0.342	0
IGKC	2.32717323409743	0.961	0.911	0
AC096579.7	2.32273732600444	0.417	0.083	0
IGLC2	2.04974723506305	0.859	0.649	0
SSR4	1.97079506462838	0.955	0.608	0
CCL3	1.88310400635241	0.401	0.1	0
MZB1	1.85548507189336	0.913	0.302	0
DERL3	1.6126125685226	0.788	0.218	0
TNFRSF17	1.59226881259828	0.754	0.186	0
XBP1	1.56586069222735	0.832	0.278	0
FKBP2	1.55991092845702	0.834	0.3	0
FKBP11	1.54096169229181	0.788	0.195	0
SEC11C	1.52537747973113	0.823	0.306	0
HSPA1B	1.52116788196665	0.638	0.225	0
DNAJB9	1.4831609904652	0.746	0.243	0
HERPUD1	1.47713815979417	0.945	0.687	0
ANKRD28	1.43804164135754	0.582	0.142	0

Cluster 4_13

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HLA-DPB1	1.19656328507929	0.971	0.702	0
HLA-DPA1	1.17926410676914	0.97	0.717	0
HLA-DRA	1.09899110387575	0.997	0.799	0
HLA-A	1.06308469559813	0.98	0.883	0
CD74	1.04515432868497	1	0.956	0
TMSB4X	0.83783304970108	1	0.992	0
ACTB	0.835791908192409	0.99	0.904	2.05119359997549e-285
HLA-DRB1	1.00847078563524	0.956	0.684	3.7159255627956e-284
HLA-DQA1	0.981670404636775	0.88	0.585	2.5758611453842e-244
HLA-DQB1	0.99982387840055	0.865	0.533	6.95764712765883e-244
LTB	1.21100639711323	0.826	0.499	1.44639280628271e-241
CD52	0.828915293832535	0.94	0.707	9.18244271228736e-193
CAPG	1.00855129835576	0.402	0.149	1.75896108443946e-180
HOPX	0.977633874748074	0.265	0.073	6.85501620850976e-158
COTL1	0.910812153837835	0.666	0.41	2.7847112386827e-150
ARL6IP5	0.864868018743337	0.516	0.278	3.39789374494364e-127
CD82	0.813838537738884	0.364	0.148	2.45129521499358e-125
HLA-DRB5	0.910790632492023	0.513	0.368	1.42187756172243e-61
S100A4	0.860703526828224	0.438	0.335	5.25463165713307e-39
AL928768.3	0.862927586086103	0.491	0.431	1.3236795264431e-26

Cluster 9_10

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
HMGB2	2.92952537169356	0.686	0.235	0
STMN1	2.92238996770972	0.58	0.133	0
RGS13	2.67405162070697	0.707	0.021	0
MARCKSL1	2.58761345936642	0.874	0.295	0
HMGN2	2.56483693461446	0.858	0.531	0
TCL1A	2.54316558006607	0.804	0.107	0
HMGN1	2.51967252650735	0.967	0.6	0
ACTG1	2.49233603781354	0.995	0.79	0
GAPDH	2.31122483742834	0.988	0.799	0
ACTB	2.2319998734064	0.998	0.906	0
LRMP	2.16101657724544	0.728	0.112	0
HMGB1	2.15665752499151	0.941	0.732	0
KIAA0101	2.05437973454933	0.457	0.03	0
HMGA1	2.02334920838714	0.771	0.224	0
PFN1	1.98504313250158	0.985	0.787	0
PTTG1	1.96114217957876	0.552	0.076	0
TUBB	2.51931732619148	0.699	0.327	1.30118165133735e-284
H2AFZ	1.9380078037232	0.796	0.55	1.57651365928373e-195
TUBA1B	2.21933887039883	0.666	0.468	1.08057408031387e-124
HIST1H4C	2.27304930241359	0.498	0.319	2.18817875859149e-61

Cluster 11_17

Gene	Average Log2FC	% Expressed in cluster	% Expressed others	p-value adjusted
IGHG3	6.58946866929302	0.824	0.317	0
IGHG1	6.48364357447739	0.814	0.284	0
IGHG2	6.38783455650974	0.665	0.077	0
IGHG4	5.98026342256049	0.789	0.25	0
IGHGP	4.24114011163245	0.354	0.007	0
MZB1	2.16615918215015	0.97	0.437	0
XBP1	1.96600273595554	0.959	0.397	0
HSP90B1	1.88139009770943	0.935	0.43	0
FKBP11	1.6078257326899	0.878	0.324	1.85020864825481e-289
ITM2C	1.60113769928362	0.776	0.271	3.69576696435799e-269
PPIB	1.7542724222688	0.867	0.45	1.16893149741511e-244
SEC11C	1.41830824137654	0.886	0.419	4.85480285846436e-234
MYDGF	1.74412574921146	0.474	0.119	2.09691565298866e-219
SUB1	1.41161685793752	0.879	0.624	4.70904176431471e-159
NEAT1	1.43043497427383	0.877	0.575	5.60162450599924e-113
IGKC	2.29274627357827	0.983	0.921	2.40377956289766e-69
IGLV6-57	1.49036739354878	0.326	0.125	1.20907014335966e-61
IGLL5	2.4318009651225	0.368	0.169	5.65050625562366e-49
IGLC3	1.97897254079235	0.745	0.615	3.70465460281764e-19
IGLC2	2.66808462629656	0.807	0.699	1.90274127994769e-17

Second annotations

Clusters	Annotation	Clusters	Annotation
0_2	Memory?	11_17	Plasma cells secreting IgG+
1_16	Naive	12	?? (IgM+)
3_5_6_8	Plasma cells secreting IgA+	14	??
4_13	Memory?	15	? (overexpression of heat-shock proteins)
7	?? (IgA+)	18	??
9_10	Plasmablast?	-	-